Abstracts of APG Vol. 56(2)

Invited article

Classification, Molecular Phylogeny, Divergence Time, and Morphological Evolution of Pteridophytes with Notes on Heterospory and Monophyletic and Paraphyletic Groups

 

MASAHIRO KATO*

 

Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan

 

Pteridophytes are free-sporing vascular land plants that evolutionarily link bryophytes and seed plants. Conventional, group (taxon)-based hierarchic classifications of pteridophytes using phenetic characters are briefly reviewed. Review is also made for recent tree-based cladistic analyses and molecular phylogenetic analyses with increasingly large data sets of multiple genes (compared to single genes in previous studies) and increasingly large numbers of species representing major groups of pteridophytes (compared to particular groups in previous studies), and it is extended to most recent analyses of estimating divergence times of pteridophytes. These classifications, phylogenetics, and divergence time estimates have improved our understanding of the diversity and historical structure of pteridophytes. Heterospory is noted with reference to its origins, endospory, fertilization, and dispersal. Finally, monophyletic and paraphyletic groups recently proposed or re-recognized are briefly described.

 

Key words: classification, divergence time estimate, ferns, heterospory, molecular phylogeny, pteridophytes.

 

 


Claytosmunda; a New Subgenus of Osmunda (Osmundaceae)

 

YOKO YATABE‚P, NORIAKI MURAKAMI‚P and KUNIO IWATSUKI‚Q

 

1Department of Botany, Graduate School of Science, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan; ‚QThe Museum of Nature and Human Activities, Hyogo, 6 Yayoigaoka, Sanda 669-1546, Japan

 

Infrageneric system of the genus Osmuda is revised and a subgenus, Claytosmunda, is proposed as a monotypic subgenus based on Osmunda claytoniana.

 

Key words: Claytosmunda, new subgenus, Osmundaceae

 

 


Thismia mullerensis (Burmanniaceae), a New Species from Muller Range, Central Kalimantan

 

HIROKAZU TSUKAYA 1,2,3* and HIROSHI OKADA4

 

1 National Institute for Basic Biology/Okazaki Institute for Integrated Bioscience, Myodaiji-cho, Okazaki 444-8585, Japan; 2 The Graduate University for Advanced Studies, Shonan Village, Hayama, Kanagawa 240-0193, Japan; 3 Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; 4 Botanical Gardens, Faculty of Science, Osaka City University 2000 Kisaichi, Katano, Osaka 576-0004, Japan.

 

A new species of the genus Thismia (Burmanniaceae), T. mullerensis, is described and illustrated from Muller Range, Central Kalimantan, Indonesia. It appears to belong to sect. Euthismia Schltr. (sect. Thismia ) in having inner perianth free, spreading and roots vermiform, creeping, and to subsect. Odoardoa Schltr., having perianth lobes equal in length and size. It most resembles T. alba that was collected in 1930 from Malay Peninsula and T. lauriana reported from Central Kalimantan in 1996, but differs in flower structure and color. This species is the third species of the genus Thismia reported from Central Kalimantan.

 

Keywords: achlorophyllous, Burmanniaceae, Central Kalimantan, flora, Malesia, new species, saprophyte, Thismia mullerensis,

 

 


Musa beccarii (Musaceae) Varieties in Sabah, Northern Borneo

 

 

MARKKU HÄKKINEN1* MONICA SULEIMAN 2 and JOHNNY GISIL 3

 

 

1University of Helsinki Botanical Garden. P. O. Box 44, Fin-00014 University of Helsinki, Finland; 2, 3 Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah. Locked Bag 2073, 88999 Kota Kinabalu, Sabah, Malaysia.

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Musa beccarii Simmonds var. beccarii is re-described based on extensive field studies in the Eastern part of Sabah, Malaysia. Then a new variety of wild banana, Musa beccarii Simmonds var. hottana Häkkinen, is described and illustrated. This extremely rare new variety of was only found in one area at the lower Kinanbatangan River, Eastern part of Sabah. It is also considered that Musa pigmaea M.Hotta nom. nud. from East Kalimantan, Indonesia, might be the synonym or a closely related variety to the new variety.

 

Key words: Borneo, Callimusa, Musa, Musa beccarii, Musa beccarii var. becariii, Musa beccarii var. hottana, Musa pigmaea, wild bananas.

 

 

Molecular Phylogenetics of Phalaenopsis (Orchidaceae) and allied Genera: Re-evaluation of Generic Concepts

 

TOMOHISA YUKAWA1, KOICHI KITA2, TAKASHI HANDA2, TOPIK HIDAYAT3 and MOTOMI ITO3

 

1Tsukuba Botanical Garden, National Science Museum, Amakubo, Tsukuba, 305-0005, Japan; 2Institute of Agriculture and Forestry, University of Tsukuba, Tennodai, Tsukuba, 305-8577, Japan; 3Graduate School of Arts and Science, University of Tokyo, Komaba, Tokyo, 153-8902, Japan.

 

Molecular phylogenetic analyses were performed using data sets derived from DNA sequences of the plastid genome (matK and trnK introns) and the nuclear genome (rDNA ITS) in an examination of relationships of all sections of Phalaenopsis and closely related genera. The following insights were provided: (1) The genera Lesliea and Nothodoritis are nested within Phalaenopsis. (2) Phalaenopsis subgenus Aphyllae and section Esmeralda, often treated as the independent genera Kingidium and Doritis respectively, are also nested within Phalaenopsis. (3) Two subgenera of Phalaenopsis, namely, Phalaenopsis and Parishianae, are not monophyletic. (4) Phalaenopsis sections Deliciosae, Stauroglottis, Amboinenses and Zebrinae are not monophyletic. (5) Inconsistencies between the plastid and nuclear lineages indicate a hybrid origin of Phalaenopsis minus and Phalaenopsis philippinensis. (6) In light of these findings, and to accommodate phylogenetic integrity and stability in nomenclature, we adopt a broadly defined Doritis characterized by the possession of four pollinia, an explicit character state.

 

Key words: Doritis, introgression, ITS, matK, molecular, Orchidaceae, Phalaenopsis, phylogenetics, trnK

 

 

Arabidopsis kamchatica (Fisch. ex DC.) K. Shimizu & Kudoh

and A. kamchatica subsp. kawasakiana (Makino) K. Shimizu & Kudoh,

New Combinations

 

KENTARO K. SHIMIZU1,2*, SHINJI FUJII3, KAROL MARHOLD4,5, KUNIAKI WATANABE6 and HIROSHI KUDOH6

 

1 Department of Genetics, North Carolina State University, 3513 Gardner Hall, Raleigh, NC 27695, USA; 2 Department of Botany, Faculty of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; 3 Division of Human Environment, University of Human Environments, Motojuku, Okazaki, Aichi 444-3503, JAPAN; 4 Institute of Botany, Slovak Academy of Science, Dúbravská cesta 14, SK-845 23 Bratislava, Slovak Republic; 5 Department of Botany, Charles University, Benátská 2, CZ-128 01 Praha 2, Czech Republic; 6Department of Biology, Faculty of Science, Kobe-University, Nada-ku, Kobe 657-8501, Japan

 

 

New combinations for two taxa, Arabidopsis kamchatica (Fisch. ex DC.) K. Shimizu & Kudoh and A. kamchatica subsp. kawasakiana (Makino) K. Shimizu & Kudoh, are proposed. The lectotypes of both names were chosen. The former taxon was previously recognized as either Arabis kamchatica, Cardaminopsis kamchatica or an infraspecific taxon under Arabis lyrata or Arabidopsis lyrata. The latter was previously recognized as either Arabis kawasakiana, Arabis lyrata subsp. kawasakiana or a synonym of Arabidopsis lyrata subsp. kamchatica. We here report the bivalent nature of meiotic chromosome of A. kamchatica subsp. kawasakiana, n = 16 II and 2n = 32. We also provide a molecular evidence of allotetraploidy using a nuclear Chalcone Synthase (CHS) sequence. In conjunction with former chromosomal and morphological studies, we considered that both taxa should be placed under the genus Arabidopsis but distinct from Arabidopsis lyrata. Arabidopsis kamchatica will provide a unique opportunity for the molecular genetic analysis of genome duplication, by utilizing the genetic and genomic information on a model species Arabidopsis thaliana.

 

Keywords: Arabidopsis, Arabis, bivalent chromosome, Cardaminopsis, CHS, kamchatica, kawasakiana, lyrata, tetraploid

 

 


Genetic Status of Angelica japonica var. boninensis (Umbelliferae) Distributed in the Bonin and the Daito Islands

 

AKIHIRO SEO1, FUYUO NOBUSHIMA2 and NORIAKI MURAKAMI1

 

1Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan; 2Ogasawara village, Tokyo 100-2101, Japan

 

Three infraspecific taxa, var. japonica, var. hirsutiflora, and var. boniensis, were recognized in Angelica japonica by Yamazaki (1990). He considered that var. boninensis is distributed both in the Bonin and the Daito Islands. In this paper, in order to evaluate taxonomic status of var. boninensis, we examined the populations of the three varieties on the basis of allozyme analysis. In the obtained UPGMA dendrogram, three major clusters of the populations from the following three areas were recognized: I) the southern part of Kyushu, the Hedo population of Okinawa Island and the Daito Islands; II) the Bonin Islands; and III) Okinawa Island and the Yaeyama Islands. The plant populations of the Daito and the Bonin Islands, which were identified as var. boninensis by Yamazaki (1990), were divided into two different clusters based on the UPGMA dendrogram. The values of genetic identities among the three clusters were lower than the mean values of those among conspecific plant populations. Differences in leaflet shape coincided with these clusters. We concluded that the clusters I, II, and III can be taxonomically treated as independent species Angelica japonica A. Gray, A. boninensis Tuyama, and A. hirsutiflora Liu, Chao et Chung, respectively.

 

Key words: allozyme, Angelica boninensis, Angelica hirsutiflora, Angelica japonica, Bonin Islands, Daito Islands, taxonomy

 

 

Molecular Phylogeny and Chromosomal Evolution of Japanese Schoenoplectus (Cyperaceae), Based on ITS and ETS 1f Sequences

 

OKIHITO YANO and TAKUJI HOSHINO

 

Department of Biosphere-Geosphere System Science, Graduate School of Informatics, Okayama University of Science, Ridai-cho 1-1, Okayama-shi, Okayama 700-0005, Japan.

 

ITS and ETS 1f sequence data were used to estimate the phylogeny of 13 Japanese Schoenoplectus species, and karyomorphological observations were made on 14 species of this genus. Two major clades were identified in the Japanese Schoenoplectus molecular phylogenetic tree: (1) one including all species of section Actaeogeton, and (2) the another comprising the two sections Malacogeton and Schoenoplectus. Phylogenetic analysis, including three published species of section Schoenoplectus, supported a monophyly of the two major clades. These molecular phylogenetic data also support the intrageneric relationships and morphological characters of genus Schoenoplectus, as defined by Smith & Hayasaka (2001). The section Actaeogeton clade and the sections Malacogeton and Schoenoplectus clade showed the same chromosomal evolution; each clade had both high and low chromosome numbers. The high chromosome numbers may arise by polyploidy because chromosome sizes were almost equal in both. Therefore, chromosomal evolution in the genus Schoenoplectus may be caused more by polyploidy more than aneuploidy. In our study, the putative natural hybrids, S. ~ trapezoideus and S. ~ uzenensis were found. The chromosome number of S. ~ trapezoideus was 2n=43 and S. ~ uzenensis was 2n=58. These two hybrids had an intermediate chromosome number of both putative parents.

 

Keywords: chromosomal evolution, Cyperaceae, Diffuse centromeric chromosome, ITS and ETS 1f phylogeny, Schoenoplectus

 


Short communication

Floral Scent Chemistry and Stamen Movement of Chimonanthus praecox (L.) Link (Calycanthaceae)

 

HIROSHI AZUMA1, MASAO TOYOTA2 and YOSHINORI ASAKAWA2

 

1 Department of Botany, Graduate School of Science, Kyoto University, Kyoto, 606-8502 Japan. 2 Faculty of Pharmaceutical Science, Tokushima Bunri University, Tokushima, 770-8514 Japan.

 

The floral scent of Chimonanthus praecox (L.) Link (Calycanthaceae; Magnoliids) was collected by the headspace method and analyzed using gas chromatography - mass spectrometry (GC-MS). The main components of the floral scents are benzyl acetate (42.2 %), trans-b-ocimene (24.8 %), linalool (17.2 %), and benzyl alcohol (6.9 %). These compounds are common in the scent profiles of many plants throughout the angiosperms. In addition, the female and male stages (phases) of the protogynous, drooping flowers of C. praecox f. concolor Makino were observed. In the female phase the stamens are bent toward the tepals away from the pistils at a right angle. After approximately two days the stamens commence to move to enclose the pistils. It takes from one to four days for the stamens to eventually enclose the pistils in the various flowers and then anthers shed their pollen.

 

Key words: fragrance, floral biology, protogynous, volatiles, Magnoliids

 

 


Short communication

Chromosome Observations in Species of Cayratia (Vitaceae). II. Intraspecific Polyploidy in C. trifolia

 

HIROSHI OKADA1, HIROKAZU TSUKAYA2, 3, 4 and MARYATI MOHAMED5

 

1Botanical Gardens, Faculty of Science, Osaka City University, 2000 Kisaichi, Katano, Osaka 576-0004, Japan; 2National Institute for Basic Biology/Center for Integrative Bioscience, Myodaiji-cho, Okazaki, Aichi 444-8585, Japan; 3The Graduate University for Advanced Studies, Shonan Village, Hayama, Kanagawa 240-0193, Japan; 4Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan; 5Institute for Tropical Biology and Conservation, Universiti Malaysia Sabah, Kota Kinabalu, Sabah, Malaysia

 

The chromosome numbers of two species of Cayratia (Vitaceae) are reported. Cayratia japonica var. mollis had a diploid number of 2n=40, while C. trifolia was found to have both diploids of 2n=40 and tetraploids of 2n=80.

 

Keywords: Cayratia japonica var. mollis, Cayratia trifolia, chromosome number, intraspecific polyploidy, Vitaceae

 



* Present address: Department of Botany, National Science Museum, Tsukuba, 305-0005, Japan.

@ This article is formed from the presentation as one of contributions for the International Symposium 2004, Asian Plant Diversity and Systematics, held at Sakura, Chiba, Japan on July 29 – August 2, 2004.

* e-mail: tsukaya@nibb.ac.jp

* Corresponding author. E-mail: markku.hakkinen@kymp.net

* correspondence should be addressed (email: kshimizu@ncsu.edu)